5-Dimethylbenzaldehyde-DNPH_Kain Industrial Additive

Background and overview[1] The 2,4-dinitrophenylhydrazine (DNPH) colorimetric method is a classic method for determining the carbonyl content of proteins. The oxidized protein carbonyl content increases, and the carbonyl group can react with 2,4-dinitrophenylhydrazine to form 2,4-dinitrophenylhydrazone, 2,4-dinitrophenylhydrazone is a reddish-brown precipitate. After dissolving the precipitate with guanidine hydrochloride, the absorbance value at 370nm can be…