Main molecular biology reagents and culture media_Kain Industrial Additives

Using the E.coli JM109 genome as a template, using pQE-nlpE-F and pQE-nlpE-R, pQE-spy-F and pQE-spy-R as primers respectively, 2 genes were amplified through high-fidelity PCR Fragments, sizes were 711 bp and 486 bp respectively. PCR program: 95°C for 5 min; 98°C for 10 s, 55°C for 15 s, 72°C for 1 min, 30 cycles; 72°C…